Fig. 4. ER-α agonist (PPT) and ER-β agonist (DPN) affect phosphorylation of kinases. (A-C, left and middle panels) Western blotting and densitometry analysis of p-ERK, p-AKT and p-P38, after treatment with PPT and DPN in RBCs from males and females. Representative blotting normalized for the GAPDH content, and histogram showing densitometry analysis of three different experiments. The agonists markedly affected the phosphorylation of these kinases in a sex-dependent manner. (A-C, right panels) Cytometric analysis. After treatment with ER agonists, the content of phosphorylated ERK, AKT and P38 decreased in RBCs from males and increased in RBCs from females. The data are the average of △% (treated vs controls).